Neural Stem Cells Increase Nerve Growth Factor in Stroke Rat Model

Stroke-induced acute brain injury is a major cause of disability and mortality. Stem cell therapy has the ability to treat the neurological damage due to stroke worldwide. In vitro, Neural Stem Cell (NSC) stimulates axon growth and exhibit protective effects through the secretion of Nerve Growth Factor (NGF). The current study aims to determine the NGF levels in rats with hypoxic ischemia given neural stem cells. An animal experimental study was used. Sixteen healthy rats were divided into two groups, namely the hypoxia group and the hypoxia-treatment group. Both groups underwent the stroke model procedure. Hypoxia group was categorized as control group that received no intervention. The hypoxia-treatment group received Neural Stem Cell (NSC) intra-ventricularly. NSC was derived from Mesenchymal Stem Cell (MSC) adipose rat tissue. In the next ۴۸ hours, the rats were then slowly sacrificed. The clinical proven stroke was assessed with neurological score. NGF levels were measured from blood serum and determined by ELISA examination. Positive expression of MSC was validated from CD ۱۰۵, CD ۹۰, and CD ۲۹. Negative expressions of MSC were validated from CD ۴۵. NSC was categorized by the Nestin expression. All rats in both groups suffered from hemiparesis with different levels based on neurology score evaluated on a six-point scale at ۲۴ hours. There was statistically significant different NGF levels between groups after NSC procedure (p<۰.۰۵). The administered NSCs in stroke rat model produced higher levels of NGF.

Keywords:

mortality , Nerve Growth Factor , Neural Stem Cell , Stroke