Preconcentration of trace copper(II)using octadecyl silica membrane disks modified Functionalized Graphene with octadecylamine (ODA–GO) in water samples and paraffinembedded tissues from Liver loggerhead turtles specimens by FAAS

A simple and reproducible method for the rapid extraction and determination of trace amounts of copper(II) ions using octadecyl-bonded silica membrane disks modified byFunctionalized Graphene with octadecylamine (ODA–GO)and Atomic Absorption Spectrometry is presented. The method is based on complex formation on the surface of the ENVI-18 DISKTM disks followed by stripping of the retained species by minimum amounts of appropriate organicsolvents. The elution is efficient and quantitative. The effect of potential interfering ions, pH, ligand amount, stripping solvent, and sample flow rate were also investigated. Under the optimal experimental conditions, the break-through volume was found to about 1000mL providing apreconcentration factor of 500. In the present study, we report the application of preconcentration techniques still continues increasingly for trace metal determinations by flame atomic absorptionspectrometry (FAAS) for quantification of Cu in Formalin-fixed paraffin- mbedded (FFPE) tissues from Liver loggerhead turtles. The maximum capacity of the disks was found to be 389± 4 μg for Cu2+.The limit of detection of the proposed method is 3ng per 1000mL.The method was applied to the extraction and recovery of copper in different water samples.

Keywords:

Copper(II) , SPE , Octadecyl slica disks , AAS , Functionalized Graphene with octadecylamine (ODA–GO) , Formalin-fixed paraffin-embedded (FFPE)