h askari - Radiation Application Research School,Nuclear Science and Technology Research Institute(NSTRI) Atomic Energy organization of IRAN (AEOI)Alborz, Iran. Department of Food Science and Technology,School of Agriculture, Shiraz University, Fars, Iran
s shahbazi - Radiation Application Research School,Nuclear Science and Technology Research Institute(NSTRI) Atomic Energy organization of IRAN (AEOI)Alborz, Iran
m bakhtiyari - Department of Food Science and Technology,School of Agriculture, Shiraz University,Fars, Iran
m moosavinasab - Department of Food Science and Technology,School of Agriculture, Shiraz University,Fars, Iran

Two strain of Trichoderma (T. viride and T. harzianum) were isolated and used for β-glucanase enzyme production. The succinoglycan was produced by fermentation of sucrose by Agrobacterium radiobacter and its chemical structural properties were investigated by TLC, FT-IR and 1H-NMR spectroscopy. The results showed that the biogum is composed of glucose and galactose units, carrying pyruvate, succinate and acetate groups and indicated the presence of succinoglycan. The highest β-glucanase activity was observed in T. harzianum. The SDS-PAGE profiles have several enzyme bonds such as β(1,3), β(1,4) and β(1,6) glucanase. The T. harzianum and T. viride have both enzyme bonds of β(1,3) glucanase and β(1,6) glucanase. Cel6A (CBH II) only was observed in T. harzianum. The high values β-succinoglycanase activity in T. harzianum due to present of β(1,3), β(1,6) glucanse enzymes and synergism that occurs between of them. Succinoglycan is a good substrate for total β-glucanase activity assay.

Trichoderma spp.; Succinoglycan; Chemical structure; β-glucanase