Determination of Oxytetracycline by RhodamineB-Modified gold nanoparticles

In this study,a rapid, economical and highly sensitive method is described for determination of trace amounts of Oxytetracycline based on the fluorescence resonance energy transfe r(FRET) between AuNPs and Rhodamine B (RB) ,in which RB acts as the donor and AuNPs as the acceptor.The reaction was monitored spectrofluorometrically by measuring the increase in fluorescence of RB at 572 nm after 4min of mixing the reagents in tris buffer solution ( pH=6.5).Various chemical (such as the type of buffer, the effect of acidity and reagents concentration ) and reaction time were studied and were optimized.By using the recommended procedure and under optimum conditions ,the calibration graphs were linear from 0.01 to 20mg.L-1of Oxytetracycline. The proposed method was applied to the determination of Oxytetracycline in milk and water samples.

Keywords:

Oxytetracycline , RhodamineB , goldnanoparticles , fluorometry