The Draft Genome Sequence of Nesterenkonia sp Strain F Isolated From Aran-Bidgol Salt Lake in Iran

Publish Year: 1390
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:

IBRC01_029

تاریخ نمایه سازی: 5 مهر 1393

Abstract:

The draft genome of the aerobic, Gram-positive,chemoorganotroph and halophilic Nesterenkoniasp strain F consists of 2,812,133 bp chromosome(AFRW01000000 accession no.). The genome projectdata is also available at GenBank under the GenomeProject ID 68173.Nesterenkonia is an aerobic, Gram-positive,chemoorganotroph, catalase positive, mesophilicand halophilic genus from Micrococcaceae family.Nesterenkonia sp strain F was isolated from Aran-Bidgolhypersaline lake in Iran and characterized to producethe halophilic and organic solvent tolerant α-amylases.Despite of its high potential for producing the variousnatural compounds and specialized mechanismsfor adaption to extreme conditions, there is a littleinformation about the genetic and genome features ofthe Nesterenkonia genus.Genome sequencing of Nesterenkonia sp strain F wasperformed using 454 GS-FLX titanium. We obtained a total201,320 random reads, covering a total of 44,347,672bp. The approximate coverage of Nesterenkonia spstrain F genome was 16 fold. The sequence reads wereassembled into 138 contigs using Newbler Assemblersoftware v. 2.3. Then, the RAST annotation pipeline wasused to annotate the DNA sequence.The G+C content of the draft genome was 71.5% thatwas similar to previous report by Stackebrandt et. al.. Thesum of 138 large contigs size of Nesterenkonia sp strainF is 2,812,133 bp. The draft genome contained 2484genes with 1794 non-hypothetical and 690 hypotheticalprotein coding sequences and 50 structural RNAs.Analysis of annotated genome sequence ofNesterenkonia sp strain F revealed the presence ofgenes involved in production of α-amylases includingmaltodextrin glucosidase and alpha-glucosidase.Nesterenkonia sp strain F comprises Clustered RegularlyInterspaced Short Palindromic Repeats (CRISPR), whichgrants an effective mechanism against foreign geneticelements as an adaptive immune system (4). The CRISPRassociatedhelicase Cas3, RecB family exonucleaseCas4b and CRISPR-associated protein Cas1 have beendetected using CRISPRFinder online program

Keywords:

Nesterenkonia sp. Strain F , Draft genome sequencing , Annotation , CRISPR

Authors

Laleh Parsa Yeganeh

Molecular Biology Division, Iranian Biological Resource Center, Tehran, Iran

Reza Azarbaijani۱

Microorganisms Bank, Iranian Biological Resource Center, Tehran, Iran

Sajjad Sarikhan

Molecular Biology Division, Iranian Biological Resource Center, Tehran, Iran.

Abolhasan Shahzadeh Fazeli

Department of Molecular Biology, Iranian Biological Resource Center, Tehran, Iran