Laleh Parsa Yeganeh - Molecular Biology Division, Iranian Biological Resource Center, Tehran, Iran
Reza Azarbaijani۱ - Microorganisms Bank, Iranian Biological Resource Center, Tehran, Iran
Sajjad Sarikhan - Molecular Biology Division, Iranian Biological Resource Center, Tehran, Iran.
Abolhasan Shahzadeh Fazeli - Department of Molecular Biology, Iranian Biological Resource Center, Tehran, Iran

The draft genome of the aerobic, Gram-positive,chemoorganotroph and halophilic Nesterenkoniasp strain F consists of 2,812,133 bp chromosome(AFRW01000000 accession no.). The genome projectdata is also available at GenBank under the GenomeProject ID 68173.Nesterenkonia is an aerobic, Gram-positive,chemoorganotroph, catalase positive, mesophilicand halophilic genus from Micrococcaceae family.Nesterenkonia sp strain F was isolated from Aran-Bidgolhypersaline lake in Iran and characterized to producethe halophilic and organic solvent tolerant α-amylases.Despite of its high potential for producing the variousnatural compounds and specialized mechanismsfor adaption to extreme conditions, there is a littleinformation about the genetic and genome features ofthe Nesterenkonia genus.Genome sequencing of Nesterenkonia sp strain F wasperformed using 454 GS-FLX titanium. We obtained a total201,320 random reads, covering a total of 44,347,672bp. The approximate coverage of Nesterenkonia spstrain F genome was 16 fold. The sequence reads wereassembled into 138 contigs using Newbler Assemblersoftware v. 2.3. Then, the RAST annotation pipeline wasused to annotate the DNA sequence.The G+C content of the draft genome was 71.5% thatwas similar to previous report by Stackebrandt et. al.. Thesum of 138 large contigs size of Nesterenkonia sp strainF is 2,812,133 bp. The draft genome contained 2484genes with 1794 non-hypothetical and 690 hypotheticalprotein coding sequences and 50 structural RNAs.Analysis of annotated genome sequence ofNesterenkonia sp strain F revealed the presence ofgenes involved in production of α-amylases includingmaltodextrin glucosidase and alpha-glucosidase.Nesterenkonia sp strain F comprises Clustered RegularlyInterspaced Short Palindromic Repeats (CRISPR), whichgrants an effective mechanism against foreign geneticelements as an adaptive immune system (4). The CRISPRassociatedhelicase Cas3, RecB family exonucleaseCas4b and CRISPR-associated protein Cas1 have beendetected using CRISPRFinder online program

Nesterenkonia sp. Strain F, Draft genome sequencing, Annotation, CRISPR