Development of disomic single-locus DNA microsatellite markers for Persian sturgeon (Acipenserpersicus) from Caspian Sea

Information about the number of discrete genetic stocksof Persian sturgeon (Acipenser persicus) is necessary formanagement of this commercially important species.Disomic DNA microsatellite markers are among the besttools for determining stock structure in fishes. However,because of the polyploid ancestry of all sturgeons,most loci exhibit more than two alleles per individual,prohibiting the use of powerful analytical methods thatassume disomic inheritance. We scored products from 37sets of microsatellite primers developed in lake sturgeon(Acipenser fulvescens) and one set of Atlantic sturgeon(Acipenser oxyrinchus oxyrinchus ) to determine whetherthey would amplify disomic loci in Persian sturgeon.Thirty six loci (95%) were successfully amplified in Persiansturgeon. We scored one monomorphic locus, 12 asbeing disomic, 19 tetrasomic, three octosomic, and oneas ambiguous. This is the first report on developmentof disomic single-locus DNA microsatellite markers forPersian sturgeon. These loci could be used to characterizevarious geographical populations of the Persian sturgeonin their native ecosystem such as the Caspian Sea.

Keywords:

Acipenser persicus , Caspian Sea , singlelocusDNA microsatellite markers