Fabrication and Optimization BSA Nanoparticles as Drug Delivery Systems
Publish place: 11th National Iranian Chemical Engineering Congress
Publish Year: 1385
نوع سند: مقاله کنفرانسی
زبان: English
View: 2,389
This Paper With 10 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
NICEC11_393
تاریخ نمایه سازی: 4 اردیبهشت 1386
Abstract:
Nanoparticle prepared by coacervation method and subsequent cross-linking of nanoparticle represent promising carrier for drug delivery. Bovine Serum Albumin (BSA) nanoparticles with a size from 78.9 to 421 nm diameter were prepared using a self-assembly simple coacervation method and cross-linking with glutaraldehyde. Nanoparticle was purified with 48,800 g centrifuge following by dialysis, micro and ultra filtration. The produced nanoparticles were subjected to physical, morphological as well as biochemical characterisation. Photographs obtained for image analysis studies by SEM and AFM proved semi-spherically shape of the particles whilst size distribution was measured by photon correlation
spectroscopy. Fourier transforms infra-red spectroscopy (FTIR) and gel electrophoresis results indicated the nanoparticles have high purity. Several process parameters were examined to achieve a suitable size of nanoparticle through one factor at a time method such as pH, temperature, BSA concentration, agitation speed, glutaraldehyde concentration, organic solvent adding rate and the ratio of BSA/ organic solvent. The minimum size of nanoparticles at de desired incubator of 4°C, pH=7.5, 30 mg.ml-1 BSA concentration and constant agitation speed of 500 rpm was obtained. The mechanistic fabrication of protein nanoparticles and their characterisation is strongly discussed.
Keywords:
Authors
Rahimnejad
MSc Student of chemical engineering Faculty of Chemical Engineering, Noshirvani Institute of Technology Mazandaran University, Babol, Iran
Jahanshahi
Associate professor Faculty of Chemical Engineering, Noshirvani Institute of Technology Mazandaran University, Babol, Iran
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :