ناشر تخصصی کنفرانس های ایران

لطفا کمی صبر نمایید

Publisher of Iranian Journals and Conference Proceedings

Please waite ..
Publisher of Iranian Journals and Conference Proceedings
Login |Register |Help |عضویت کتابخانه ها
Paper
Title

Characterization process parameters influencing the productivity of human interferon-γ in recombinant E.coli cultivations

Year: 1385
COI: NICEC11_418
Language: EnglishView: 1,995
This Paper With 10 Page And PDF Format Ready To Download

Buy and Download

با استفاده از پرداخت اینترنتی بسیار سریع و ساده می توانید اصل این Paper را که دارای 10 صفحه است به صورت فایل PDF در اختیار داشته باشید.
آدرس ایمیل خود را در کادر زیر وارد نمایید:

Authors

Valiolah Babaeipour - PhD student in chemical engineering-biotechnology
Seyed Abbas Shojaosadati - Prof. in industrial biotechnology
Rasoul Khalilzadeh - Assist. prof. in chemical engineering-biotechnology
Nader Maghsoudi - Assoc. prof. in molecular and cellular biology

Abstract:

The effect of various process parameters on over-expression of human interferon-γ sing an IPTG inducible expression system in recombinant Escherichia coli BL21 (DE3) [pET3a-ifnγ] was investigated. Process parameters include: i) type of cultivation process (batch and fed-batch), ii) feeding strategy (in fed-batch), iii) feed composition in postinduction feeding, and iv) induction conditions, were applied and then overall productivity of interferon-γ was examined. It was found that overall productivity of Interferon-γ was significantly affected by all of the studied parameters, but specific growth rate at both pre and post-induction, type of cultivation process and feeding strategy are more effective. By applying the fed-batch cultivation, feeding strategy with maximum attainable specific growth rate throughout the process, optimum induction conditions, and suitable composition in post-induction feeding basis of amino acid composition of recombinant protein and E.coli, overall productivity and concentration of rhIFN-γ was increased from 0.044 to 3 g l-1 h-1(interferon-γ), and 0.31 (at batch culture with LB medium) to 51 g l-1, (at f ed-batch culture with simple medium) respectively. Also overall productivity and concentration of biomass was increased of 0.14 to higher than 7.3 g l-1 h-1 (dry cell weight) and 0.95 to over than 127 g l-1 (dry cell weight), respectively. According to available data this is the highest specific yield and productivity that reported for recombinant proteins production yet.

Keywords:

Paper COI Code

This Paper COI Code is NICEC11_418. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/30705/

How to Cite to This Paper:

If you want to refer to this Paper in your research work, you can simply use the following phrase in the resources section:
Babaeipour, Valiolah and Shojaosadati, Seyed Abbas and Khalilzadeh, Rasoul and Maghsoudi, Nader,1385,Characterization process parameters influencing the productivity of human interferon-γ in recombinant E.coli cultivations,11th National Iranian Chemical Engineering Congress,Tehran,https://civilica.com/doc/30705

Research Info Management

Certificate | Report | من نویسنده این مقاله هستم

اطلاعات استنادی این Paper را به نرم افزارهای مدیریت اطلاعات علمی و استنادی ارسال نمایید و در تحقیقات خود از آن استفاده نمایید.

New Papers

Share this page

More information about COI

COI stands for "CIVILICA Object Identifier". COI is the unique code assigned to articles of Iranian conferences and journals when indexing on the CIVILICA citation database.

The COI is the national code of documents indexed in CIVILICA and is a unique and permanent code. it can always be cited and tracked and assumed as registration confirmation ID.

Support