Immobilization of pectinase on Fe3O4 nanoparticles coated with Docusate sodium salt

Nanomagnetic particles of Fe3O4 were synthesized by co-precipitation method using NH4OH as precipitating reagent and N2H4.H2O as an oxidation-resistant reagent and finally coated with docusate sodium salt (AOT) as an anionic surfactant. These nanoparticles present a very simple, mild, low-priced and time-saving process for enzyme immobilization. The immobilized pectinase could be recovered by magnetic separation and retained 36.6 and 43.33% of activity after 4 recycles at 40 and 50 °С, respectively. It shows less stability at high temperature

Keywords:

Magnetic nanoparticles (MNPs) , AOT , Pectinase , Enzyme immobilization