Computational Fluid Dynamics simulation of a single cell of shaken Microtiter plate for determination of Air/Water interface shape

Shaken microtiter plates have recently attracted tremendous attraction in HTS (high-throughput screening) experiments. Detailed engineering analysis of such systems therefore is required to allow for precise estimation of oxygen transfer rate (OTR) followed by hydrodynamics. To accomplish, one may need to develop computational schemes which will help determine key engineering parameters i.e. shear stress distribution, velocity profile and interfacial area in smallscale bioreactors. CFD (computational fluid dynamics) techniques as an invaluable tool can foster scientists understanding of crucial parameters incorporated in miniaturized bioreactors. Consequently,Simulation efforts leads to comprehensive understanding of detailed fluid flow in orbital shaking vessels which are then compared in two different geometries at shaking frequency of 100 rpm.

Keywords:

Orbital Shaking , Hydrodynamics , Microtiter plates , Computational fluid dynamics