Identification of Salmonella spp. and Salmonella typhimuriumby a multiplex PCR-based assay from poultry carcasses inMashhad- Iran

Poultry meat has been identified as one of the principal foodborne sources of Salmonella.In this preliminary study the prevalence of Salmonella spp. and its typhimurium serovarcontamination of broiler carcasses, were determined. Using the rinse test method, numbers of60 samples, representing 20 broiler flocks, were collected from poultry carcasses after thechilling stage in the processing line at a commercial broiler slaughtering facility in Mashhad,Iran. The presence of Salmonella spp and Salmonella typhimurium in collected samples wereassessed by performing the pre-enrichment and enrichment culture, followed by multiplex-PCR assay. The primers were selected from the invA and fliC genes, specific for the detection ofSalmonella spp. and Salmonella typhimurium, respectively. In this study 8.3% and 1.6% ofpoultry carcasses were found to be contaminated with Salmonella spp and Salmonellatyphimurium respectively. In order to provide a more accurate profile of the prevalence ofSalmonella spp and Salmonella typhimurium in broiler carcasses, it is pertinent to use multiplex-PCR method that could be considered as an appropriate alternative to conventional culturemethod.

Keywords:

poultry , Salmonella spp , Salmonella typhimurium , multiplex PCR