Salmonella typhimurium in Natrix natrix: detection andidentification by culture and multiplex PCR methods

Publish Year: 1390
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_IJVM-5-4_009

تاریخ نمایه سازی: 26 اردیبهشت 1394

Abstract:

A water snake (Natrix natrix) was referred to the Small AnimalTeaching Hospital, Faculty of Veterinary Medicine, University ofTehran. It had two subcutaneous masses. Radiographic and ultrasonographicimages revealed the presence of two subcutaneous abscesses. Theabscesses were removed surgically and specimens were examined byconventional microbial culture and multiplex PCR. Inv-A universalprimers were selected for detection of Salmonella at genus level. Inorder to identify Salmonella tphimurium, specific primers of Rfbj, Fljband Flic related on genes sequences of O4, H1:i and H2:1,2,respectively, were used. In the positive control for expected size, PCRproducts were amplified from the fliC, inv-A, fljB and rfbJ genes.According to the results, this study showed antigens can be useful fordetecting and identifying Salmonella typhimurium and can beachieved by using specific primers of O4, H1:i and H2:1,2 antigen,because only S. typhimurium has this antigenic structure out of about2668 Salmonella serovars.

Authors

A Rostami

Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

T Zahraei Salehi

Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran,Tehran, Iran.

M.M Dehghan

Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.

M Masoudifard

Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.