Anovel chemical method for measuring ketone bodies insubclinical ketosis
Publish place: Iranian Journal of Veterinary Medicine، Vol: 6، Issue: 1
Publish Year: 1391
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJVM-6-1_002
تاریخ نمایه سازی: 26 اردیبهشت 1394
Abstract:
BACKGROUNDS: Subclinical ketosis can cause greater economic loss due to a lack of clinical symptoms. OBJECTIVES: The present study was aimed to design a chemical method for measuring serum ketone bodies in the affected subclinical cows. METHODS: Acetoacetate concentrations were measured using a nitroprusside reaction and β-hydroxy butyric acid (BHBA) which was oxidized to acetoacetate using nanosilver particles to determine its concentration. Recovery tests were done for different concentrations of betahydroxy butyrate in bovine pooled serum. RESULTS: Actoacetate levels were in range of 0.1-6 mM, and the values for BHBA were found to be in the range of 0.125-3 mM. CONCLUSIONS: It was concluded that this technique can be considered as a simple method for measuring ketone bodies in biological fluids
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Authors
H Gholampour
Animal Biological Products Research Group, Iranian Academic Center for Education,Culture and Research(ACECR). Jahad-e-Daneshgahi, Tehran Unit, Tehran, Iran.
P Asadian
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Lorestan, Khoramabad, Iran
A Erfanmanesh
Animal Biological Products Research Group, Iranian Academic Center for Education,Culture and Research(ACECR). Jahad-e-Daneshgahi, Tehran Unit, Tehran, Iran
M Jahantigh
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Zabol, Zabol, Iran