A Kamali Sangani - Graduated from the Faculty of Agriculture, University of Tarbiat Modares, Tehran, Iran
A.A Masoudi - Department of Animal Sciences, University of Tarbiat Modares, Tehran, Iran
S.A Hosseini - Department of Animal and Poultry Nutrition, Animal Sciences Research Institute, Karaj, Iran

BACKGROUND: The mucus layer plays an important role as an intermediate for the protection of the gut against acidic chyme, digestive enzymes, and pathogens; in addition, it acts as a lubricant and facilitator of nutrient transportation. Phytogenic compounds seem to promote intestinal mucus production. OBJECTIVES:The current study was conducted to investigate the effects of low and high levels of energy and amino acids in combination with turmeric, thyme, and cinnamon on chickenperformance and expression of mucin 2 gene. METHODS: The eight experimental groups consisted of diluted and condensed diet with and without the addition of 5g/kg of each turmeric, thyme, and cinnamon to the diet. Chicken performance was recorded. Expression analysis of the mucin 2 gene was carried out by quantitative RT-PCR. RESULTS: Body weight gain, feed intake, FCR, and mortality rate were not affected by diets (p>0.05). A significant (p<0.05) reduction of the mucin 2 geneexpression was observed in chickens fed by condensed diet; however, the expression increased by supplementation of turmeric, thyme, and cinnamon. CONCLUSIONS: These results, in addition to the function of herbs in increasing the activity of some enzymes which is possibly related to the mucin biolo gical pathways, showed that the application of turmeric, thyme, and cinnamon could be useful in poultry diets. It appears that supplementation of turmeric, thyme, and cinnamon couldincrease mucin 2 gene expression in the small intestine, and this can improve intestinal digestive function and defense

broiler chicken, cinnamon, mucin 2gene expression, thyme, turmeric