Fluorescence quenching of human serum albumin by polyacrylamide grafted onto kappacarrageenan capped CdS quantum dots

The synthesis of water-soluble quantum dots (QDs) is of crucial importance for nanobiotechnology. Apolyacrylamide grafted onto kappa-carrageenan was successfully synthesized and employed for coating CdS QDs inaqueous solution. The as-obtained QDs were characterized by transmission electron microscopy (TEM). After that, theinteraction of coated QDs with human serum albumin (HSA) were investigated under the simulative physiologicalconditions. Fluorescence analysis at different temperatures revealed the gradual quenching of both proteins by titrationwith coated QDs. The binding sites number and binding constant were calculated at various temperatures.

Keywords:

Fluorescence quenching , HSA , CdS quantum dots , Stern–Volmer