Fluorescence study on the interaction of bovine serum albumin with a novel CdS quantum dots coated by poly (acrylic acid) grafted onto starch

Interactions between CdS quantum dots coated by poly (acrylic acid) grafted onto starch (CdS-PAA-g-starchQDs) and bovine serum albumin (BSA) were investigated. CdS-PAA-g-starch QDs were synthesized in aqueous solutionand characterized by UV–vis and fluorescence spectrum. Fluorescence spectrum was used to detect the interactionsbetween as-prepared CdS-PAA-g-starch QDs and protein molecules. The interaction parameters, including bindingconstant (Ka), binding site number (n) and quench constant (Kq), were determined by fluorescence spectrum. The resultsobtained here analyze the biosafety of CdS QDs in terms of the biological behavior of biomolecules and could serve asbasis for the application of CdS QDs to bioscience.

Keywords:

Fluorescence quenching , BSA , Quantum dots , starch