Application of nano particles in removal of microbial pollutants

seudomonas aeruginosa is a microorganism resistant to chlorine and UV-C irradiation. TiO2 photo catalytictechnology can destroy bacteria, which are resistant to oxidative destruction of cell membrane caused by sole UVirradiation. This study aims to investigate the total mineralization of the bacterium (aeruginosa) to the extent of death andcell-mass destruction using TiO2 photo catalytic oxidation process. In this work the effects of parameters such as amountof TiO2, irradiation time, initial concentration of bacterium, presence of ascorbic acid and effect of cell wall on removalof aeruginosa were studied. The data, which were obtained in this study, showed that the optimum concentration of TiO2was 325 ppm. Also at the initial concentration of TiO2 equal to 325 ppm and initial microorganism MPN / 100 ml equalto 300 and after 75 min UV irradiation time, P. aeruginosa removal efficiency was 94.3 %. Removal efficiency ofaeruginosa in the absence of TiO2 or UV irradiation was very low. Decreasing the concentration of microorganismsincreased its removal efficiency. Removal efficiency of spheroplast cells was more than intact cells of aeruginosa, whichshows the important role of cell wall on cell resistance against chemical agents. Ascorbic acid had inhibitory effect onthis process.

Keywords:

AOP , Disinfect , photo catalytic , seudomonas aeruginosa