G Khayati - Department of Chemical Engineering, Technical Faculty, University of Guilan, Rasht, Iran
M Zamani - Department of Chemical Engineering, Technical Faculty, University of Guilan, Rasht, Iran

The aim of this study was the use of fish waste hydrolysate (FWH) as a substrate for alkaline protease production and the enzyme production kinetics using isolated Bacillus sp. in a batch system. The results show that with the addition of FWH to the fermentation medium with a final concentration of4% (optimal concentration), alkaline protease value reached a maximum value (89 U/ml), which is 63% higher than that of the control medium. Several kinetic models were evaluated; the combinationof the Moser and Boulton kinetic model gave the best prediction. The results show that a goodagreement between the model solutions and the experimental data for cell, substrate concentrations and enzyme activity was obtained, and this nonlinear mathematical model performed satisfactorily on biomass, substrate, and enzyme predictions. Also, the quadratic correlation coefficients obtained were higher than 0.98.Therefore, the kinetic model then may be used to simulate the fermentation variables

Alkaline Protease , Fish Waste Hydrolysate , Modeling , Bacillus