Effect of Nitate Reductase enzyme amount on synthesis of silver nanoparticles

Nowadays, synthesis of silver nanoparticles is very common because it has many applications in different fields. The synthesis of these nanoparticles is done by means of physical, chemical, or biological methods. However, due to its inexpensive and environmentally friendly features, the biological method is more preferable than the other two methods. In the present study, using nitrate reductase enzyme available in the Escherichia coli bacterium (E.coli), the biosynthesis of silver nanoparticles was investigated and the effect of enzyme amount on nanoparticles synthesis were studied. Finally, the produced nanoparticles were studied by means of uv-vis Spectrophotometer, Dynamic Light Scattering (DLS) technique, and Transmission electron microscopy (TEM) .

Keywords:

Silver nanoparticles , Biosynthesis , Nitrate Reductas , DLS analysis , TEM