PCR DEVICE WITH INTEGRATED THERMAL CYCLING AND FLUORESCENCE DETECTION ELEMENTS
Publish place: 13th Iranian Conference on Electric Engineering
Publish Year: 1384
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
ICEE13_044
تاریخ نمایه سازی: 27 آبان 1386
Abstract:
In this paper, we present both a theoretical evaluation and the fabrication of a novel polymerase chain reaction (PCR) microdevice. This microdevice contains elements for both thermal cycling and fluorescence detection. The proposed device is composed of a reaction chamber with integrated temperature sensor, heaters, p-n diode and optical filter. The advantage of combining these in a single structure is that real time detection of DNA amplification will be possible using a small volume of the PCR solution. The photodiode is covered by a thin film optical filter in order to block out the light which is used to excite the fluorophore dyes. CdS is used for the first time for such a filter and the complete micro-fabrication process is described
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Authors
Bahrami
University of Southampton, School of Electronics and Computer Science, Highfield, Southampton, SO۱۷ ۱BJ, United Kingdom
Melvin
University of Southampton, School of Electronics and Computer Science, Highfield, Southampton, SO۱۷ ۱BJ, United Kingdom
Wilkinson
University of Southampton, School of Electronics and Computer Science, Highfield, Southampton, SO۱۷ ۱BJ, United Kingdom
Evans
University of Southampton, School of Electronics and Computer Science, Highfield, Southampton, SO۱۷ ۱BJ, United Kingdom
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