Mohamad Bokaeian - Infectious Diseasec and Tropical Medicin Research Center Zahedan University of Medical Scienes,Zahedan, Iran
Mousa Sheikh - Department of Microbiology, Medical faculty, Kerman university of medical science, Kerman, Iran
Zahra Shahi - Department of Microbiology, Kerman Science and Research Branch, Islamic Azad University, Kerman,Iran.
Saeide Saeidi - Department of Microbiology, Kerman Science and Research Branch, Islamic Azad University, Kerman,Iran.

The purpose of this study was to examine the evolution of antimicrobial activity of flower extract of Hibiscus sabdariffal against antibiotic-resistant Escherichia coli and Staphylococcus aureus that isolates from the urinary tract infection by microtiterplate method. All 42 strains 30 E.coli and 12 S. aureusisolated from urine culture of hospitalized patients in Zabol ( Zabol, south-eastern Iran) suffered from urinary tract infections during the years 2011- 2012 were evaluated and the extract of Hibiscus sabdariffal obtained by rotary and the minimum inhibitory concentrations were investigated to characterize theantimicrobial activities of this extract. The result show that E. coli were resistance to 4 of the agent and more resistance to tetracyclin(63.3%), erythromycin(56.6%) and cefixime(40 %), S.aureus more sensitive were antibiotic resistance to vancomycin and cefixime. The highest MIC values was found to be 20mg/ml against two E.coli. The leas MIC values was found to 1.25 mg/ml against three S.aureus

Hibiscus sabdariffal, Antibacterial activity, Human pathogen