Fereshteh javadian - Zabol Medicinal Plant Research Center, Zabol University of Medical Sciences, Zabol, Iran.
Shahla Sahraei - Zabol University of Medical Sciences, Zabol, Iran.
Arezoo Azizi - Zabol University of Medical Sciences, Zabol, Iran.

Infectious diseases are responsible for thousands of worldwide early death .Many commercial antibiotics have been used to control human's infectious diseases all over the world. Calotropis procera Linn a wild growing plant of Asclepiadaecae family is known to possess multifarious medicinal properties. Total 80 non repetitive clinical E. coli strains recovered during 7 month, and were screened for ESBL production by disc diffusion test, and the MIC and MBC of Calotropis procera chlorophyll extract against ESBL positive E. coli isolates were determined. The results showed a total of 30 of 80 (37.5%) isolates harboured ESBL enzymes. Calotropis procera Calotropis procera extract were effective against ESBLproducing E. coli isolates. There is need for a correct and reliable phenotypic test to identify ESBL betalactamases and also these bioactive plants may help alleviate the problem of drug resistance. Thepresence of these chemical constituents in this plant is an indication that the plant, if properly screened using additional solvents, could yield drugs with pharmaceutical significance.

Escherichia coli, Calotropis procera, Extract, Antibiot resistant