ناشر تخصصی کنفرانس های ایران

لطفا کمی صبر نمایید

Publisher of Iranian Journals and Conference Proceedings

Please waite ..
Publisher of Iranian Journals and Conference Proceedings
Login |Register |Help |عضویت کتابخانه ها
Paper
Title

Arabidopsis leaf plasma membrane proteome using a gel free method: Focus onreceptor–like kinases

Year: 1391
COI: JR_JPMB-1-1_004
Language: EnglishView: 342
This Paper With 10 Page And PDF Format Ready To Download

Buy and Download

با استفاده از پرداخت اینترنتی بسیار سریع و ساده می توانید اصل این Paper را که دارای 10 صفحه است به صورت فایل PDF در اختیار داشته باشید.
آدرس ایمیل خود را در کادر زیر وارد نمایید:

Authors

H Najafi Zarrini - Laboratory of Plant Physiology, Centre for Ecological and Evolutionary Studies (CEES), University of Groningen, theNetherlands
F Fusetti - Membrane Enzymology, Department of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute(GBB), University of Groningen, the Netherlands
J.T.M Elzenga - Laboratory of Plant Physiology, Centre for Ecological and Evolutionary Studies (CEES), University of Groningen, theNetherlands
F.C Lanfermeijer - Seed and Production Physiology Research, Syngenta Seeds B.V., P.O. Box 2, 1600 AA, Enkhuizen, the Netherlands

Abstract:

The hydrophobic proteins of plant plasma membrane still remain largely unknown. For example in theArabidopsis genome, receptor-like kinases (RLKs) are plasma membrane proteins, functioning as the primaryreceptors in the signaling of stress conditions, hormones and the presence of pathogens form a diverse familyof over 610 genes. A limited number of these proteins have appeard in protein profiles. The detection of theseproteins and thus the determination of their dynamics and tissue specificity, is technically challenging due totheir low abundance and association to a lipid membrane. To identify new putative membrane proteinsespecially receptor systems, we used a gel free proteomic strategy based on mass spectrometry analyses of aplasma membrane fraction enriched in hydrophobic proteins. We produced from Arabidopsis leaf a highlypurified plasma membrane fraction with the aqueous two-phase partitioning technique. By separating theproteins in the plasma membrane fraction with ion exchange and reverse phase chromatography and analyzingthe resulting fractions on a MALDI-TOF mass spectrometer, over 900 proteins were detected. The plasmamembrane proteome generated by this approach contains numerous plasma membrane integral proteins, onethird displaying at least four trans-membrane segments. An in silico analysis shows a correlation between theputative functions of the identified proteins and the expected roles for plasma membrane in transport,signaling, cellular traffic and metabolism. Of these proteins, 304 were annotated as membrane proteins, 69were RLKs, distributed among the different receptor families in proportions reflecting the distribution in thegenome. Of the RLKs that were identified, most are reported for the first time at the protein level and willconstitute interesting targets for further functional studies.

Keywords:

Paper COI Code

This Paper COI Code is JR_JPMB-1-1_004. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/450318/

How to Cite to This Paper:

If you want to refer to this Paper in your research work, you can simply use the following phrase in the resources section:
Najafi Zarrini, H and Fusetti, F and Elzenga, J.T.M and Lanfermeijer, F.C,1391,Arabidopsis leaf plasma membrane proteome using a gel free method: Focus onreceptor–like kinases,https://civilica.com/doc/450318

Research Info Management

Certificate | Report | من نویسنده این مقاله هستم

اطلاعات استنادی این Paper را به نرم افزارهای مدیریت اطلاعات علمی و استنادی ارسال نمایید و در تحقیقات خود از آن استفاده نمایید.

New Papers

Share this page

More information about COI

COI stands for "CIVILICA Object Identifier". COI is the unique code assigned to articles of Iranian conferences and journals when indexing on the CIVILICA citation database.

The COI is the national code of documents indexed in CIVILICA and is a unique and permanent code. it can always be cited and tracked and assumed as registration confirmation ID.

Support