The effect of medium and plant growth regulators onmicropropagation of Dog rose (Rosa canina L.)

Dog rose (Rosa canina L.) is one of the most important ornamental and medicinal plants which are usedas a rootstock for ornamental roses such as Rosa hybrid and Rosa floribunda. In vitro propagation of rosehas a very important role in rapid multiplication of species with desirable traits and in production ofhealthy and disease-free plants. Micropropagation of Rosa canina L. was revised, using its nodalsegments under different combinations of BAP (0, 0.5, 1, 1.5 and 2 mgl⁻1), GA3 (0 and 0.5 mgl⁻1) andNAA (0 and 0.5 mgl⁻1) on Murashige and Skoog (MS) and Van der Salm medium (VS) in proliferationstage and using different combinations of NAA and IBA (0, 0.3, 0.6 and 0.9 mgl⁻1) and ½ VS medium inrooting stage. The highest shoot proliferation was obtained on VS medium containing 2 mgl⁻1 BAP.Furthermore, the highest root induction obtained in ½ VS containing 0.6– 0.9 mgl⁻1 of NAA or IBA. Thepresent study presents an in vitro protocol for R. canina.

Keywords:

Micropropagation , Native plants , Plant growth regulators (PGRs) , Rosa canina L