Isolation, molecular cloning and expression analysis of Aeluropuslittoralis Monodehydroascorbate reductase (MDHAR) gene under saltstress

Plants are naturally exposed to frequent changes in environmental conditions such as salt stress affectingtheir growth and development, and consequently a considerable reduction in total productivity.Monodehydroascorbate reductase (MDHAR), a key enzyme in ascorbate-glutathione cycle, serves as animportant antioxidative enzyme in scavenging of reactive oxygen spices (ROS). In this study, MDHARgene from Aeluropus littoralis was isolated using specific primers by RT-PCR and its expressionvariation was investigated at 0, 150, 300, 450 and 600 mM of NaCl through Real-time PCR. The resultsindicated that A. littoralis MDHAR gene compromised 1436 bp without any introns, showing a highsimilarity with Sorghum bicolor. The only alteration of MDHAR expression was observed at 300 mMNaCl, i.e. cytosolic MDHAR in both shoot and root was increased 1.3 and 1.4 fold compared to controlsamples. It could be concluded that accumulation of MDHAR reduces at Nacl concentrations higher than300 mM.

Keywords:

Aeluropus littoralis , Ascorbate-glutathione cycle , MDHAR , Real-Time PCR , ROS