Azadeh Mohseni - Department of Plant Breeding, Sari Agricultural Sciences and Natural Resources University,Sari, Iran
Ghorban Ali Nematzadeh - Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciencesand Natural Resources University, Sari, Iran
Ali Dehestani - Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciencesand Natural Resources University, Sari, Iran
Behzad Shahin - Genetics and Agricultural Biotechnology Institute of Tabarestan, Sari Agricultural Sciencesand Natural Resources University, Sari, Iran

Plants are naturally exposed to frequent changes in environmental conditions such as salt stress affectingtheir growth and development, and consequently a considerable reduction in total productivity.Monodehydroascorbate reductase (MDHAR), a key enzyme in ascorbate-glutathione cycle, serves as animportant antioxidative enzyme in scavenging of reactive oxygen spices (ROS). In this study, MDHARgene from Aeluropus littoralis was isolated using specific primers by RT-PCR and its expressionvariation was investigated at 0, 150, 300, 450 and 600 mM of NaCl through Real-time PCR. The resultsindicated that A. littoralis MDHAR gene compromised 1436 bp without any introns, showing a highsimilarity with Sorghum bicolor. The only alteration of MDHAR expression was observed at 300 mMNaCl, i.e. cytosolic MDHAR in both shoot and root was increased 1.3 and 1.4 fold compared to controlsamples. It could be concluded that accumulation of MDHAR reduces at Nacl concentrations higher than300 mM.

Aeluropus littoralis, Ascorbate-glutathione cycle, MDHAR, Real-Time PCR, ROS