Haleh Vaez - Drug Applied Research Centre, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Pharmacology & Toxicology, Tabriz University of Medical Sciences, Tabriz, IranStudent research Committee, Faculty of Pharmacy, Tabriz University of Medical Scie
Mojgan Arab - Drug Applied Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran Department of Pharmacology & Toxicology, Tabriz University of Medical Sciences, Tabriz, Ir
Abbas Delazar - Drug Applied Research Centre, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Pharmacognosy, Tabriz University of Medical Sciences, Tabriz, Iran
Solmaz Asnaashari - Drug Applied Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran

Eremostachys laciniata, having rich flavonoid content, is expected to have a considerable antioxidant effect. In this study We used ACMS (Accelerated cytotoxic or protective mechanism screening technique) to evaluate the possible antioxidant effect of E. laciniata rhizome against oxidative cell damages induced by different types of oxidative stress such as iron-8-hydroxyquinolin (IQ) complex and copper in freshly isolated liver cells. The extracts were prepared with n-hexane, dichloromethane and methanol. Hepatocytes were isolated from male Sprague-Dawley rats by a two-step collagenase perfusion. Cell viabilitywas measured by trypan blue exclusion method. DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay was used to evaluate the antioxidant activity. ROS formation was measured by using DCFDA (2, 7-dichlorofluorescin diacetate) probe, mitochondrial membrane potential (MMP) was assessed by rhodamine 123 fluorescence and lipid peroxidation was determined by thiobarbituric acid reactive substances (TBARS) assay. TheMET extract was demonstrated to possess a significant radical scavenging activity (RC50%=0.212). UnlikeMET extract, the n-hexane and dichloromethane extracts showed toxic effects in cell suspensions. The MET extract significantly decreased cell death and ROS formation induced by IQ complex and copper and demonstrated protective effects against copper-induced mitochondrial membrane potential collapse and lipid peroxidation. The protection induced by MET extract can be attributed to antioxidant characteristics of the phenylethanoids content.

Eremostachys Laciniata, Oxidative stress, Hepatocyte, Iron-quinolin, Copper