Ali.Asghar Karkhane - Ntional Institute of Genetic Engineering and Biotechnology. NIGEB
Ferdous Rastgar Jazii - Ntional Institute of Genetic Engineering and Biotechnology. NIGEB
Ashrafaddin Sokhannsangb - Department of Biology, Faculty of Sciences, University of Tehran

Moderately and halophilic bacteria are known to grow well in the environments with high salt concentration. In order to investigate osmo tolerance in moderately halophiles; Paenibacillus sp. strain XII was chosen and grown in the media with different concentration of salt (NaCl), total protein was extracted and analyzed by electrophoresis. A new polypeptide with molecular weight of 21.4 kDa was observed to be expressed by the bacterium in response to salt. The polypeptide was purified and isoelectric point (pI) of which was determined which indicates the protein to be highly acidic. N-terminal sequencing was carried out and sequence homology search was done. A strong homology was found between N-terminally sequenced polypeptide and a segment from the N-terminus of spore coat–associated protein; CotN of Oceanobacillus iheyensis an extremely halotolerant of deep-sea.