Random amplified polymorphic DNA analysis of Trichomonas vaginalis isolates in Tabriz-Iran

Trichomonas vaginalis is the causative agent of human trichomoniasis. It is the most common nonviral sexually transmitted disease. The infection may be asymptomatic or may cause severe vaginitis and cervicitis in women. Despite its high prevalence little is known about its genetic variability and factors’ leading to asymptomatic infections.The random amplified polymorphic DNA (RAPD) technique is a simple method to detect DNA polymorphism. We performed RAPD method with 4 different Random primers (OPD1, OPD2, OPD3, and OPD5) for typing the 120 isolates of Trichomonas vaginalis in Tabriz. Phylogenetic analysis was performed using SPSS program and dendrogram with two distinct clusters was constructed. The asymptomatic isolates tended to form a cluster, separate from symptomatic isolates. Further studies for better understanding the relationship is suggested

Keywords:

Trichomonas vaginalis , RAPD , Polymorphism