Two-Dimensional Electrophoresis Protein Profiling Of BHK Cells Infected By Rabies Virus Utilizing a Prefractionation Method

Proteomic approaches to biological research that will prove the most useful and productive require robust,sensitive and reproducible technologies for both the qualitative and quantitative analysis of complex protein mixtures. Prefractionation of complex protein mixtures is an efficient method in order to increase the separation power of twodimensional electrophoresis (2DE). We were utilized Reverse Phase High Performance Liquid Chromatography (RPHPLC) for prefractionation followed by 2DE. A step gradient elution of cell lysate was improved to yield properly resolved fractions for subsequent analysis by 2DE. Utilizing this prefractionation step showed improved resolving power up to 20 percents.