Agroinfiltration of human growth hormone in Medicago sativa and Trifolium alexanderium leaves

Using agro-infiltration, we have transiently expressed human Growth Hormone (hGH) in Medicago sativa and Trifolium alexanderium leaves. The presence of hGH was detected in leaves extracts by western blot and ELISA. The plant leaf types were effective factor in expression level of produced recombinant protein. Medicago leaves infiltrated for 30 minutes could be good candidate for this production system, but the expression rate in trifolium levaes was lower in 25 minutes that was the best time. .It could be discussed for the hydrolytic or toxic compounds that could be find in trifolium leaves.

Keywords:

Transient Expression , Molecular Farming , Agro-infiltration , Biopharmaceuticals