Modeling and simulation of growth kinetics for recombinant E. coli BL21 (DE3) producing hIFN-γ in batch and fed-batch cultures

The use of stirred tank bioreactors with automatic control of the culture environment is the most suitable technique to evaluate bacterial or fungal kinetics and the specific growth rate is a key parameter for representation of dynamic behavior of microorganisms during fermentation. Modeling and simulation of the growth kinetics of E. coli BL21 (DE3) producing hIFN-γ in batch and fed-batch cultures were studied in our research. Five different growth kinetics models were investigated using batch experimental data for determination of models parameters. The statistical parameter of was used as a criterion for kinetics model selection. The lowest value of indicates the Tessier is the best growth kinetics model. Finally, simulation results of batch and fixed volume fed batch with exponential feeding strategy were compared with experimental data. T-test evaluation was shown, there is not any significant difference between experimental and simulation results.

Keywords:

Modeling , simulation , Fed-batch culture , Interferon-γ