Optimization of Simultaneous Production of Amylase, Pectinase and Cellulase, by Aspergillus oryzae

Production of enzymes is a well studied subject however, simultaneous optimization of production of two or more enzymes and trying to develop a single condition for their optimum production, is not well documented in the literatures. Taguchi method was used for optimization of such a culture condition. In the preliminary tests, maximum production of cellulase, pectinase and amylase was determined, using wheat bran, citrus pectin and corn starch flour as substrates, respectively. Media with mixed carbon sources, containing different ratios of wheat bran, citrus pectin and corn starch were designed and the best condition for highest amount of production of these enzymes was detected. As a result, after performing the Taguchi method, an increase of 209.28%, 287.13%, 282.61% and 271.43% in the amount of produced amylase, pectinase, CMCase and FPase was showed, respectively. As the next step, a combinatory optimum point, which was an arithmetic average point of the previous points, was chosen, in which in relation to the initial conditions, the production of the mentioned enzymes was increased 185.79%, 201.32%, 160.87% and 242.86%, respectively.

Keywords:

Amylase , Aspergillus oryzae , Cellulase , Optimization , Pectinase