Co-culture of human skin keratinocytes and melanocytes on a novel biodegradable scaffold

Publish Year: 1386
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:

ICHEC05_512

تاریخ نمایه سازی: 7 بهمن 1386

Abstract:

Extensive full-thickness burns require replacement of both epidermis and dermis. Using developed procedures for establishing confluent, stratified layers of cultured human keratinocytes on the surface was generated biodegradable scaffold that contains fibroblasts. In present study, we developed culture methods for propagating of keratinocytes and fibroblasts isolated from human neonatal foreskin. The growth, proliferation and differentiation of normal human keratinocytes were evaluated in serum-free (KGM) and our modified medium. Characterization of human keratinocytes was determined using by pan-keratin and anti-involucrin monoclonal antibodies. We presented modified method using freeze-gelation to fabricate of novel biodegradable and biocompatible collagen-chitosan porous scaffolds with improved biostability. For generating of organotypic coculture epidermal keratinocytes were plated onto the upper surface of our scaffold containing embedded fibroblasts. In conclusion, these data indicate that the integration of scaffold with cocultured keratinocyte and fibroblast in our model provides a potential source of skin for grafting.

Authors

Seyed Ramin Pajoum Shariati

Biotechnology group, Chemical Engineering Department, , Amirkabir University of Technology, Tehran, Iran

Mohammad Ali Shokrgozar

National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran

Manouchehr Vossoughi

Biomedial group, Chemical Engineering Department, , Sharif University of Technology, Tehran, Iran

Mohammad Jafar Abdekhoaie

Biomedial group, Chemical Engineering Department, , Sharif University of Technology, Tehran, Iran

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  • Nangia, A., Gambhir, R., Maibach, H., ،#Factors influencing the performance ...
  • Martin, P., ،Wound healing-aiming for perfect skin regeneration, Science, 276, ...
  • Cuono, C.B., Langdon, R., Birchall, N., Barttelbort, S., McGuire. J., ...
  • Bannassch, H., Fohn, M., Unterberg, T., Bach, A.D., Weyand, B.., ...
  • design of scaffolds for use in Theء، 11. Yang, S., ...
  • Rheinwald, J.G., Green, H., *Serial cultivation of strains of human ...
  • Lauer, G., ، Autografting of feeder-cell free cultured gingival ep ...
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  • Wilkins LM, Watson SR, Proskey SJ, et al. «Development of ...
  • replacements. The Skin؛، 16. Carins, B.A., deSerres, S., Peterson, H.D., ...
  • Eaglstein, W.H., Iriondo, M., Laszlo, K., 4A composite skin substitute ...
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