Ghavamaldin Asadian - Department of Medicinal Plants, Tonekabon Branch, Islamic Azad University,Tonkabon, Iran
Aptin Rahnavard - Department of Medicinal Plants, Tonekabon Branch, Islamic Azad University,Tonkabon, Iran
Khalil Pourshamslan - Department of Chemistry, Tonekabon Branch, Islamic Azad University,Tonkabon, Iran
Mansour Ghorbanpour - Department of Medicinal Plants, Faculty of Agriculture and Natural Resources, Arak University, Iran

Hypericum perforatum L. is among important herbs worldwide, which growing wild in Iranvarious areas and altitudes particularly north of the country. However, according to differentregional and genetic reasons, its secondary metabolites differ in its growth vegetative.Therefore, in order to define its best vegetative area, the most important biochemicalcomponents in five regions and three altitudes and four samples from each regions (60samples all together) were sampled and analyzed. Experiment revealed that the highest valuesof hypericin (0.251% DW) and total phenols (412 mg/l) were obtained in Jannat Roodbarregion in a height of 1218 m and the highest amounts of flavonoid (73 μg/ml) and carotenoid(0.13 mg/ml) were observed in Pole Zangoole region in a height of 2300 m. Regressionequations have been demonstrated to have a positive linear relationship between hypericinand total phenols contents. While among hypericin and flavonoid as well as carotenoid, thisrelation forms a second degree equation. The relationship of hypericin and total phenolscontents was a negative correlation as well.

Hypericum perforatum L., Biochemical Components, Variation , Hypericin,Totalphenol, Flavonoid