Evaluation of Erythromycin – resistant on Agrobacterium vitis Among Environmental Isolates in Urmia of Iran

Agrobacterium vitis is III biovar of Agrobacterium tumefacien and making Gall on grapes bulk. Grape is one of so important fruits in Azerbaijan and spatially in Urmia so finding the best way for controlling and contrasting with grape diseases is very necessary for microbiologists and farmers. Gall is one of these diseases that can make a lot of damages. The plasmid of these pathogens can coded growth hormones genes and cause tumorsAims: the purpose of this study was to determine the prevalence of erythromycin – resistant Agrobacterium vitis isolated from environmental samples in Urmia vineyards.Research method: From May to June 2014, 20 samples isolated from Vineyards of five grape cultures in Urmia, west of Iran. After identification of Agrobacterium vitis by biochemical, microbiological and molecular methods, antibiotic susceptibility testing was performed by Kirby-Bauer disk diffusion test for this antibiotic.Results: the highest resistance was seen on all samples of Agrobacterium vitis cultures. The results showed that the frequency of erythromycin resistance is very high in Agrobacterium vitis strains isolated from vineyards that had crown Gall in west of Urmia. Thus, urgent interventions are needs to keep the emergence and transmission of these isolates to a minimum

Keywords:

Agrobacterium vitis , Erythromycin , Urmia , Iran