Raheleh Ahmadzadeh Ghavidel - Ph.D Scholare of Agroecology, Ferdowsi University of Mashhad
Samaneh Zokae - MSc of Plant Pathology, Ferdowsi University of Mashhad

Curculigo orchioides is a perennial herb of the family Hypoxidaceae. It is an endangered plant speciesof medicinal importance. In this study a simple and efficient protocol for in vitro micropropagation ofCurculigo orchioides. Through shoot tip and nodal portion explants and callogenesis of Curculigoorchioides through leaf, node and explants was used. A completely randomized design was followedfor the experiment multiple shoots were obtained on all concentrations of BAP and NAA, but BAP (1mg/l) showed the best response (90 and 80%) from both explants. Similarly the best callus responsewas observed on MS medium supplemented with 2, 4- D + Kin (1+1 mg/l) in all explants. Thenplantlets were transferred to different potting media in green house for acclimatization at the end of athree-month period, an average of 125 plants were obtained from a single meristem.

Curculigo orchioides, micropropagaton, Plant hormones, tissue culture