Faezeh Khalilian - Department of Chemistry, College of Basic Science, Yadegar -e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran
Donya Vafaei - Department of Chemistry, College of Basic Science, Yadegar -e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran
Leila Hajiaghababaei - Department of Chemistry, College of Basic Science, Yadegar -e-Imam Khomeini (RAH) Shahre Rey Branch, Islamic Azad University, Tehran, Iran

The purpose of this research is to prepare a molecularly imprinted polymer (MIP) using iron oxide (Fe3O4). Firstly Fe3O4 was and then SiO2 was coated on Fe3O4 using sol- gel technique. Prior to polymerization the vinyl group was introduced on to the surface of SiO2/Fe3O4 through chemical modification with γ - methacryloxypropyl trimethoxysilence (γ -MAPS), which can direct selective polymerization on the surface. Then a novel composite of MIP-SiO2/Fe3O4 was obtained by copolymerization in presence of vinyl group functionalized SiO2/Fe3O4, Chloramphenicol, methacrylic acid and ethylene glycol dimethacrylate. The MIP-SiO2/Fe3O4 composite was characterized by FT-IR, SEM methods. Following this product was used for analytical sample preparation based on magnetic solid phase extraction (SPE) using molecularly imprinted polymer as new synthesized sorbet for isolation and extraction of Chloramphenicol followed by HPLC-UV from honey sample. The method showed good linearity in the range of 40-500 μg/L. Furthermore, satisfactory relative recovery was obtained for honey sample

Iron oxide, Molecularly imprinted polymer, Chloramphenicol, Magnetic solid phase extraction, Honey sample