Ahmad M. Ragab Shalaby - Department of Oncologic Pathology, Damanhour National Medical Institute, Damanhour, El-Behera,Egypt.
Ahmad Mostafa Shalaby - Department of Obstetric and Gynecology, Damanhour National Medical Institute, Damanhour, El-Behera, Egypt.
Saed M. Naguib - Department of Clinical Pathology and Immunology, Damanhour National Medical Institute,Damanhour, El-Behera, Egypt.

Background: There is mounting evidence for HPV involvement in cervical cancer Human Papilloma Virus DNA is detected by hybridization techniques in 75 – 100% of patients withcondylomas, precancerous cervical dysplasia, and invasive carcinoma. Objective: The aim of this study was investigating factors that may contribute to falsenegative colposcopic biopsy results in positive high-risk HPV DNA results. Material and Methods: Patients positive for high-risk human papillomavirus (HPV)DNA with negative cervical histopathologic findings were examined between January2004 and August 2006. Results: Patients with atypical squamous cells of undetermined significance (ASC) in Papanicolaou smears, with positive HPV DNA results, but negative cervicalhistopathologic findings accounted for 4.5% of all ASC smears submitted for HPV DNA testing. We found 4% of the cases had focal HPV infection or mild dysplasia.When serial sectioning of the biopsy material were examined, we found that 29% hadclinically significant lesions: HPV infection or cervical intraepithelial neoplasia CIN 1, 18%; CIN II/III, 8%; and dysplasia, not otherwise specified (which we can notcategorize into any group), 3%. Of the remaining patients, follow-up revealed squamous abnormalities in 25%. About 5% of patients with positive HPV DNA results had anegative follow-up biopsy result. False-negative biopsies accounted for one third of cases.Conclusion: In almost one third of cases, clinically significant lesions were found when additional levels were examined.

Cervix, Dysplasia, Hybrid,Human papilloma virus