Adverse effects of ciprofloxacin on testis apoptosis and sperm parameters in rats
Publish Year: 1387
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJRM-6-2_004
تاریخ نمایه سازی: 16 شهریور 1395
Abstract:
Background: Ciprofloxacin is a commonly prescribed antibiotic in the treatment of genitourinary tract infection. Objective: The aim of this study was to investigate the effects of ciprofloxacin on testisapoptosis and sperm parameters in rat. Materials and Methods: Twenty male Wistar rats were selected and randomly dividedinto two groups; control (n=10) and experimental (n=10). The experimental group was orally received 12.5 mg/kg ciprofloxacin daily for 60 days and the control group justreceived water and food. Rats were then killed and sperm removed from cauda epididymis and analyzed for sperm motility, morphology, and viability. Testis tissues were also removed and prepared for TUNEL assay to detect apoptosis. Results: Results showed that ciprofloxacin significantly decreased the sperm concentration, motility (p<0.05) and viability (p<0.001). In addition, ciprofloxacintreatment resulted in a significant decrease in the number of spermatogenic cells (spermatogonia, spermatocyte, spermatid and sperm) in the seminiferous tubules whencompared with the control group. The apoptotic germ cells per seminiferous tubular cross section was significantly increased in the experimental group (15.11±3.523) ascompared with the control group (7.3±0.762) (p<0.05). Conclusion: It is concluded that ciprofloxacin has the toxicological effects on reproductive system in male rats.
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Authors
Arash Khaki
Department of Veterinary Pathology, Islamic Azad University of Tabriz, Tabriz, Iran.
Mahnaz Heidari
Avesina Research Center, Tehran, Iran.
Marefat Ghaffari Novin
Cellular and Molecular Biology Research Center, Shaheed Beheshti University M.C., Tehran, Iran.
Amir Afshin Khaki
Department of Anatomical Science, Tabriz University of Medical Science, Tabriz, Iran.