Detection of aromatase in human endometrial tissue cultured in three-dimensional fibrin matrix in vitro

Background: Endometriosis is the presence of endometrial glandular and stromal cells outside of the uterine cavity. Our previous study showed that in vitro culture of humanendometrial tissue in a three-dimensional (3D) fibrin matrix could mimic the early stages of endometriosis with invasion, gland and stroma formation and sprouting of newvessels. Objective: The objective of the present study was to evaluate the expression ofaromatase in in vitro cultured endometrial fragments.Materials and Methods: Endometrial samples obtained from the fundus of the uterine cavity of ten normal ovulating women on cycle days19-24 that referred to Toronto Center for Advanced Reproductive Technology (T.C.A.R.T) for infertility treatment andcultured in vitro by a three-dimensional fibrin matrix culture system. Cultures were performed in 24-well culture plates. Results: After 4 weeks, the tissues were fixed and blocked for immunostaining. Theywere stained by anti-aromatase antibodies. Although a fine anti-glycodelin positiveglandular epithelium and anti-cyclooxygenase-2 (COX-2) positive new vessels were detected and reported in our previous study, we were unable to detect any aromataseactivity in the cultured fragments. Conclusion: No aromatase activity was detected in the 3D cultured endometrial tissue.Although it is reported that aromatase is expressed in endometrial disease, it is possibly not expressed in this phase in normal endometrium.

Keywords:

Endometriosis , In vitro culture , Three-dimensional culture , Aromatase