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Title

Characterization of mesenchymal stem cells from human dental pulp, preapical follicle and periodontal ligament

Year: 1392
COI: JR_IJRM-11-3_008
Language: EnglishView: 370
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Authors

Ali Reza Navabazam - Department of Oral and Maxillo-facial Surgery, Dental School, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Fatemeh Sadeghian Nodoshan - Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Mohammad Hasan Sheikhha - Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Sayyed Mohsen Miresmaeili - Yazd Academic Center of Education, Culture and Research Higher Education Institute, Yazd, Iran

Abstract:

Background: Human dental stem cells have high proliferative potential for self-renewal that is important to the regenerative capacity of the tissue. Objective: The aim was to isolate human dental pulp stem cells (DPSC), periodontal ligament stem cells (PDLSC) and periapical follicle stem cells (PAFSC) for their potential role in tissue regeneration. Materials and Methods: In this experimental study, the postnatal stem cells were isolated from dental pulp, preapical follicle and periodontal ligament .The cells were stained for different stem cell markers by immunocytochemistry. To investigate the mesenchymal nature of cells, differentiation potential along osteoblastic and adipogenic lineages and gene expression profile were performed. For proliferation potential assay, Brdu staining and growth curve tests were performed. Finally, all three cell types were compared together regarding their proliferation, differentiation and displaying phenotype. Results: The isolated cell populations have similar fibroblastic like morphology and expressed all examined cell surface molecule markers. These cells were capable of differentiating into osteocyte with different capability and adipocyte with the same rate. PAFSCs showed more significant proliferation rate than others. Reverse transcriptase PCR (RT-PCR) for nanog, oct4, Alkaline phosphatase (ALP) and glyceraldehydes-3-phosphate dehydrogenease (GADPH) as control gene showed strong positive expression of these genes in all three isolated cell types. Conclusion: PDLSCs, DPSCs and PAFSCs exist in various tissues of the teeth and can use as a source of mesenchymal stem cells for developing bioengineered organs and also in craniomaxillofacial reconstruction with varying efficiency in differentiation and proliferation.

Keywords:

Dental pulp , Preapical follicle , Mesenchymal stem cell , Periodontal ligament stem cells

Paper COI Code

This Paper COI Code is JR_IJRM-11-3_008. Also You can use the following address to link to this article. This link is permanent and is used as an article registration confirmation in the Civilica reference:

https://civilica.com/doc/488866/

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Navabazam, Ali Reza and Sadeghian Nodoshan, Fatemeh and Sheikhha, Mohammad Hasan and Miresmaeili, Sayyed Mohsen,1392,Characterization of mesenchymal stem cells from human dental pulp, preapical follicle and periodontal ligament,https://civilica.com/doc/488866

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Type of center: علوم پزشکی
Paper count: 3,504
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