Marjan Omidi - Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Mohammad Ali Khalili - Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Research and Clinical Centerfor Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Hossein Nahangi - Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
Sareh Ashourzadeh - Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Background: In vitro maturation (IVM) is a promising treatment option for certain infertile women. Nowadays, with the aid of PolScope, it has become possible toevaluate zona pellucida (ZP) characteristics as a parameter of oocyte quality. Moreover, quality of oocytes can be influenced by many factors, such as patient’sage. The PolScope system is a non-invasive technique to assess birefringent structures such as the meiotic spindle and ZP in living oocytes.Objective: The aim was to determine the influence of the woman's age on ZP birefringence, a sign of oocyte quality, and morphology of in-vitro matured humanoocytes using non-invasive polarized light (PolScope) microscopy. Materials and Methods: ZP birefringence and morphology were determined in 105retrieved oocytes from 58 women undergoing ICSI in two age groups (≥30 years and <30 years). The immature oocytes were selected and after IVM, the quality ofmetaphase ΙΙ (MII) oocytes was assessed. The oocytes abnormalities were classifiedas intracytoplasmic and extracytoplasmic abnormalities. Results: Oocyte maturation rates were significantly reduced in ≥30 year’s women(56%) in comparison with other age group (80.7%). In addition, the ZP birefringence was significantly higher in MII oocytes in the younger groupcompared with the older group (76.2% vs. 38.1%; p=0.00). Following morphologic assessment, the rates of oocytes with extracytoplasmic (p=0.02) and bothabnormalities (extra- and intracytoplasmic) (p=0.01) were higher in aged versus the younger women. Conclusion: There was a positive relationship between advanced maternal age withdecreased ZP birefringence and oocyte morphological quality in in-vitro matured human oocytes.

ZP birefringence, Morphology, Human oocytes, Female age