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The impact of alpha lipoic acid on developmental competence of mouse vitrified pre-antral follicles in comparison to those isolated from vitrified ovaries

عنوان مقاله: The impact of alpha lipoic acid on developmental competence of mouse vitrified pre-antral follicles in comparison to those isolated from vitrified ovaries
شناسه ملی مقاله: JR_IJRM-12-1_008
منتشر شده در شماره 1 دوره 12 فصل January در سال 1393
مشخصات نویسندگان مقاله:

Sahar Hatami - School of Biology, Damghan University, Damghan, Iran
Saeed Zavareh - School of Biology, Damghan University, Damghan, Iran. . Institute of Biological Sciences, Damghan University, Damghan, Iran
Mojdeh Salehnia - Department of Anatomy, Tarbiat Modares University, Tehran, Iran.
Taghi Lashkarbolouki - School of Biology, Damghan University, Damghan, Iran.. Institute of Biological Sciences, Damghan University, Damghan, Iran

خلاصه مقاله:
Background: Cryopreservation of ovarian tissues and pre-antral follicles is a promising prospect for preservation of women fertility. Objective: The aim of this study was to evaluate the in vitro developmental competence of mouse vitrified pre-antral follicles in comparison to isolated pre-antral follicles derived from vitrified ovaries in the presence of alpha lipoic acid (ALA). Materials and Methods: Pre-antral follicles derived from fresh, vitrified-warmed ovarian tissues and vitrified–warmed pre-antral follicles were cultured individually with or without ALA, followed by adding hCG to induce ovulation. The follicle growth, oocyte maturation, and embryo development were assessed. Results: The diameter and development of follicles, oocyte maturation and embryo development rates were significantly higher in ALA supplemented groups compared to the respective ALA-free conditions groups. Aforementioned parameters were significantly higher in vitrified-warmed follicles in comparison to follicles derived from vitrified-warmed ovaries. Conclusion: These findings support a superior performance of pre-antral follicles when vitrified rather than when isolated from vitrified ovaries with regard to increasing the rates of developmental parameters. Moreover, ALA improves the in vitro maturation of pre-antral follicles in vitrified and non-vitrified samples.

کلمات کلیدی:
Vitrification, Ovary, Preantral follicles, Alpha Lipoic Acid

صفحه اختصاصی مقاله و دریافت فایل کامل: https://civilica.com/doc/488975/