Saccharin consumption increases sperm DNA fragmentation and apoptosis in mice

Publish Year: 1393
نوع سند: مقاله ژورنالی
زبان: English
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JR_IJRM-12-5_002

تاریخ نمایه سازی: 16 شهریور 1395

Abstract:

Background: Saccharin is an artificial non-caloric sweetener that used to sweeten products such as drinks, candies, medicines, and toothpaste, but our bodies cannotmetabolize it. Sodium saccharin is considered as an important factor in tumor promotion in male rats but not in humans.Objective: The objective of this study was to investigate the effect of saccharin consumption on sperm parameters and apoptosis in adult mice.Materials and Methods: Totally 14 adult male mice were divided into 2 groups.Group 1 served as control fed on basal diet and group 2 or experimental animalsreceived distilled water containing saccharin (0.2% w/v) for 35 days. After that, the left cauda epididymis of each mouse was cut and placed in Ham’s F10. Swimmedoutspermatozoa were used to analyze count, motility, morphology (Pap-staining) and viability (eosin-Y staining). Sperm DNA integrity, as an indicator of apoptosis,was assessed by SCD (sperm chromatin dispersion) and terminal deoxynucleotidyltransferase (TUNEL) assay.Results: Following saccharin consumption, we had a reduction in sperm motility with respect to control animals (p=0.000). In addition, the sperm count diminished(17.70±1.11 in controls vs. 12.80±2.79 in case group, p=0.003) and the rate of sperm normal morphology decreased from 77.00±6.40 in control animals into 63.85±6.81 in saccharin-treated mice (p=0.001). Also, we saw a statistically significant increasein rates of sperm DNA damage and apoptosis in experimental group when compared to control one (p=0.001, p=0.002 respectively).Conclusion: Saccharin consumption may have negative effects on sperm parameters, and increases the rate of sperm DNA fragmentation and apoptosis in mice.

Authors

Marzieh Rahimipour

Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Ali Reza Talebi

Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Department of Andrology,Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Morteza Anvari

Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Department of Andrology,Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Abolghasem Abbasi Sarcheshmeh

Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran