Marzieh Mahdavipour - Cell and Molecular Research Centre, Qazvin University of Medical Sciences, Qazvin, Iran..Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Farah Idali - Reproductive Immunology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran
Saeed Zarei - Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Saeed Talebi - Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran

Background: Recurrent pregnancy loss (RPL) is a multifactorial disorder. Environmental factors and genetics can affect pregnancy outcomes.Objective: Conflicting data suggest an association between estrogen receptor alpha (ESR1) gene polymorphisms and RPL. In this study, such association was investigated in Iranian women with RPL.Materials and Methods: In this case control study, blood samples were collected from 244 women with a history of three or more consecutive pregnancy losses and 104 healthy women with at least two live births. Using polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP), we studied -397C/T and -351A/G polymorphisms on ESR1 gene in case and control subjects.Results: The genotypic frequencies of -397C/T and -351A/G polymorphisms on ESR1were not significantly different between RPL and control groups (p=0.20 and p=0.09, respectively). A significantly negative correlation was observed between -397C/T and -351A/G (r=-0.852, p<0.001) in RPL women and complete linkage disequilibrium between the investigated polymorphisms was found (D’: 0.959; r-square= 0.758, p<0.001).Conclusion: This investigation suggests that the analyzed polymorphisms on ESR1gene are not associated with an increased risk of RPL in the studied population.

Estrogen receptor, Polymorphism, Abortion, Recurrent