Comparison of two Genotyping methods of HCV ;RT-PCR and direct sequencing in kermanshah province ,western Iran
Publish place: سومین کنفرانس بین المللی علوم و مهندسی
Publish Year: 1395
نوع سند: مقاله کنفرانسی
زبان: English
View: 654
This Paper With 16 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
ICESCON03_370
تاریخ نمایه سازی: 16 شهریور 1395
Abstract:
Hepatitis C virus (HCV) is an important human pathogen that can cause acute and chronic hepatitis, liver cirrhosis, and, possibly, hepatocellular carcinoma.HCV has been classified into 6 major genotypes and many subtypes.Sevral methods have been developed for HCV genotyping and direct sequencin is gold standard method.In this study collected 180 samples of patients infected with HCV were referred to Reference laboratory in Kermanshah University of Medical Sciences, for HCV genotype testing and samples were genotyped by direct sequencing of Coregene.Among them , 138 samples (76.6%) was 3a genotype, 35 (19.4%) was 1a genotype, 3 (1.7%) was 1b genotype and 4 (2.2%) was 3a and 1b .HCV was transmitted by different routes such as intravenous drug abuse (IVDA), tattooing, sexual, blood transfusion and other risk factors.This study revealed that 3a is the most prevalent genotypes in Kermanshah province and IVDA is the main risk factor in this province. Results of genotyping by RT-PCR was confirmed by direct sequencing .
Keywords:
Authors
Reza HATAMI-MOGHADAM
Central Medical Laboratory of Kermanshah, Kermanshah University of Medical Sciences, Kermanshah, Iran
Reza ALIBAKHSHI
Department of Biochemistry, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
Babak SAYYAD
Department of Infectious diseases, Kermanshah University of Medical Sciences, Kermanshah, Iran
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :