Anti-metastatic effect of Polysaccharide Extracted from Brittle star Ophiocoma erinaceus on human cervicalcancer cells in vitro

Cervical cancer is one of the most common causes of gynecologic malignancies around theworld. Unfortunately, there are severe side effects and chemo-resistance that limit the use ofdrugs in cancer treatment. Brittle stars are the most abundant group of echinoderm related tosea star which contains numerous bioactive metabolites. In the present study, we evaluatedanti-metastatic effect of polysaccharide extracted from the brittle star on HeLa cancer cells.HeLa cells were cultured in RPMI 1640 medium supplemented with 10% FBS, 1% antibiotic.After overnight, cells were treated with brittle star organic extract at different concentrations (0to 200 μg) and cultured for 24, and 48 h. Then MTT assay, DAPI, Acridine orange /propodium iodide staining and scratch assay was performed. Based on biological assays,isolated polysaccharide from brittle star suppressed HeLa cell growth with IC50concentration of (40 Âμg/ml) mostly through apoptotic recruitment. In vitro scratch assayexhibited that cell migration was successfully blocked up to 40 Âμg/ml of extractedpolysaccharide which indicating anti-metastatic effect of brittle star isolated polysaccharideon cervical cancer cells. Consequently, extracted polysaccharide from brittle star exertedcytotoxicity on cervical cancer cells can potentially prevent cervical cancer progression andmigration which demonstrate anti-cancer efficacy of marine bioactive compounds.

Keywords:

Cervical cancer , Apoptosis , Polysaccharide , Brittle star , Metastasis