Effect of Herbal Extract of Polygonum Aviculareon Lipocalin 2 Expression After EMF Exposure inMouse Testis

Introduction: Electromagnetic radiation has irreparable biological effects on varioustissues including testis. The use of antioxidants can greatly reduce the effects of thisradiation and has anti-oxidant properties but their mechanism is not clear. Therefore, inthe present study, protective effects of herbal extract of polygonum aviculare which are richin antioxidants components examined on the expression levels of lipocalin 2.Methods: Twenty four male mice, 8 weeks divided to 4 groups (one control and threeexperimental groups). Control group didn’t receive EMF exposure. EMF group micereceived 3 mT EMF during 2 months, 4 hours daily and 5 days weekly. Polygonum avicularegroup received 50 mg/kg herbal extract during 2 months and poly -EMF group received3 mT EMF during 2 months, 4 hours daily and 5 days weekly and 50mg/kg herbal extractduring 2 months. After 2 months the mice sacrificed by cervical dislocation and samplesprepared from the testis and immunofluorescent techniques was used to determinelipocalin 2 expression levels.Results: the result showed that lipocalin 2 expression was decreased in EMF group andincreased in mice which had received herbal extract of polygonum aviculare.Conclusions: As seen in the results, herbal extract increased lipocalin 2 expressionand might act as a protective agent against free radicals induced by the action ofelectromagnetic field. Although, further studies are needed to acquire more knowledgeabout the mechanisms of these extracts.

Keywords:

EMFPolygonum aviculareLipocalin 2Testis